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(PDF) S100A4 Is A Biomarker And Regulator Of Glioma Stem ...
*P < 0.05 has indicated that S100A4 could be a novel marker and regulator of glioma stem cells in human and murine ma- lignant gliomas [5]. Different expression levels of S100A4 lead to different ... Retrieve Doc

Lipofectamine RNAiMAX Reagent | Thermo Fisher Scientific - US
With its ability to transfect a broad range of cell types, Lipofectamine RNAiMAX reagent, combined with the Invitrogen BLOCK-iT Alexa Fluor Red Fluorescent Control, gives you the most versatile approach to all of your knockdown experiments (Table 1, Figure 3).Lipofectamine RNAiMAX offers a simple protocol—no need to remove transfection complexes, or change or add medium following transfection. ... Fetch Here

Knockdown Of TAZ Modifies Triple-negative Breast Cancer Cell ...
Transfection was done with Lipofectamine™ RNAiMA x (Life Technologies) regent according to the manufacturer's protocol. The regions of the TAZ mRNA (GenBank accession no. NM_001168278) and YAP mRNA (GenBank accession no. NM_006106) were selected as the RNAi target sites. The RNA interfering sequences were 5'-CCGuuuCCCuGAuu ... Fetch Full Source

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Aggiudicatario/Beneficiario;Codice Fiscale;Partita Iva;Descrizione;Classificazione;Struttura;Responsabile;Importo;Valuta;Data Inizio;Data Fine;Estremi conferimento ... Document Viewer

S100A4 Interacts With P53 In The Nucleus And Promotes P53 ...
S100A4 interacts with p53 in the nucleus. (a) Fluorescence microscopy images of S100A4 subcellular location in untreated A549 cells in relation to f-actin staining (phalloidin) and non-muscle ... Get Document

Breast Milk-Transforming Growth Factor-β2 Specifically ...
Immunostaining was performed by incubation with p-ERK antibody (Cell Signalling Technologies, Mass., USA) diluted 1:100 for 60 min followed by incubation with Vectastain Universal Elite ABC kit with 3,3′-diaminobenzidine as chromogen for 60 min employing the protocol and reagents recommended by the manufacturer for frozen sections. ... Read More

Control #1) were obtained from Life Technologies and was transfected individually into cells at 20 nM of concentrations using Lipofectamine RNAiMA X (Invitrogen) according to the manufacturer’s instructions. Western blotting analysis Whole cell lysates were subjected to SDS-PAGE, and proteins were transferred to PVDF membranes (GE Healthcare). ... Read Full Source

Lipofectamine Reagent | Thermo Fisher Scientific - US
Lipofectamine reagents offer you the ability to consistently transfect many cell types with high efficiency and exceptional ease of use. Our Lipofectamine reagent–specific protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types (see "Download protocol" links in the table below). ... Get Document

Mosquito And Drosophilaentomobirnaviruses Suppress DsRNA- And ...
Life Sciences, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands, 2Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands and 3Institute of Virology, University of Bonn Medical Centre, Sigmund Freud Str. 25, 53127 Bonn, Germany Received July 8, 2013; Revised May 27, 2014; Accepted May 28, 2014 ... Get Content Here

2012聚焦转染：首站InvitrogenIInvitrogennvitrogen 编者按2005年，生物通曾重磅推出“聚焦转染”专题，详细介绍了各大公司的转染试剂， ... Retrieve Here

PDF Hosted At The Radboud Repository Of The Radboud ...
PDF hosted at the Radboud Repository of the Radboud University Nijmegen The following full text is a publisher's version. For additional information about this publication click this link. ... Get Doc

Bioz Ratings For Life-Science Research
For c-Jun silencing experiments, a specific Silencer Select predesigned siRNA was purchased from Life Technologies Corp., along with a Silencer negative-control siRNA. siRNA transfection efficiencies were determined by using the Block-iT Alexa Fluor red fluorescent control (Life Technologies Corp.) as a positive control. .. Transfection ... Fetch This Document

Lipofectamine 2000（Invitrogen）产品说明书-资料下载-上海恪敏生物科技有限公司
Lipofectamine® 2000转染试剂是一种多用途转染试剂，可在各种贴壁和悬浮细胞系中提供高效转染。研究人员可使用Lipofectamine® 2000试剂进行基于siRNA和shRNA的基因敲除实验及基因表达研究。Lipofectamine® 2000是Life Technologies提供的用于 ... Read Full Source

Lipofectamine® RNAiMAX Reagent - Thermo Fisher Scientific
Life Technologies Subject: Lipofectamine® RNAiMAX Transfection Reagent is a proprietary formulation for transfecting small RNAs \\(e.g., siRNA, Silencer® Select siRNA, Stealth® RNAi, mirVana miRNA mimics and inhibitors\\) into a wide range of eukaryotic cells. ... Read Full Source